![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K3906002:Design
Designed by: Ruyi Shi Group: iGEM21_ASTWS-China (2021-10-14)
MHETase-SpyTag
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 332
Illegal PstI site found at 1093 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 332
Illegal PstI site found at 1093 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 332
Illegal PstI site found at 1093 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 332
Illegal PstI site found at 1093 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
For strong MHETase expression, MHETase was inserted into pET-21a(+) vector and we did codon optimization for successful E. coli expression. In order to make METase and PETase effectively coupled, SypCatcher and SpyTag system was used.
Source
The sequence was based on BBa_K3039004.