Composite

Part:BBa_K3906002:Design

Designed by: Ruyi Shi   Group: iGEM21_ASTWS-China   (2021-10-14)


MHETase-SpyTag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 332
    Illegal PstI site found at 1093
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 332
    Illegal PstI site found at 1093
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 332
    Illegal PstI site found at 1093
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 332
    Illegal PstI site found at 1093
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For strong MHETase expression, MHETase was inserted into pET-21a(+) vector and we did codon optimization for successful E. coli expression. In order to make METase and PETase effectively coupled, SypCatcher and SpyTag system was used.


Source

The sequence was based on BBa_K3039004.

References